Audited Supplier
D2111
/
/
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Research Use Only
Classic
For Biological Purpose, For Microbiological, Research Use Only
Standard
Laboratory Reagents, Teaching Reagents
Laboratory Produced
Chemical Reagent
Research Use/for Lab
Biochemical Reagent
Magen Biotech
Box
100 Preps/Kit
China
3822009090
Product Description
Product Name HiPure Gel Pure DNA Mini Kit
Cat. No. & Specifications D2111,100Preps/kit
Introduction
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR,sequencing,restriction digestion, orvarious labeling reactions. In addition,this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Main Composition
| Product | D2111-01 | D2111-02 | D2111-03 |
| Purification times | 20 Preps | 100 Preps | 250 Preps |
| Buffer GDP | 20 ml | 120 ml | 250 ml |
| Buffer DW2 | 6 ml | 2 x 20 ml | 2 x 50 ml |
| Elution Buffer | 1.5 ml | 20 ml | 60 ml |
| HiPure DNA Mini Columns I | 20 | 100 | 250 |
| 2 ml Collection Tubes | 20 | 100 | 250 |
Storage conditions and Validity
HiPure Gel Pure DNA Kits components are guaranteed for at least one year when stored at room temperature.If any precipitates form in the buffers,warm at 37ºC to dissolve.
Applicable Instrument
Nucleic Acid Extraction Machine such as KingFisher Flex, Kingfisher Duo or Similars. Automatic Nucleic acid workstation such as Tican, Hamilton, Aurora, BGI or similars.
Sample Requirements
Virus DNA/RNA was extracted from whole blood, serum, plasma, diseased materials, feces and body fluid. If the volume of liquid sample less than 200µL, add PBS buffer or saline to 200µL.
Preparation before Use
- According to the label, add 1.25ml(96 preps), 2.5ml(200 preps) Protease Dissolve Buffer Blue into the bottle of PK/Carrier RNA, then stored at - 20oC after dissolve.
- Dilute Buffer MW1 with 34ml (96 preps), 67ml (200 preps) absolute ethanol as shown on the label and store at room temperature.
- Prepare 150ml (96 Preps) or 250ml (200Preps) 80% Ethanol using Absolute Ethanol and Nuclease-free Water, and store at room temperature.
96-Channel Nucleic Acid Extractor Operation (KingFisher Flex)
- Add the Reagents/sample to the well of f the deep well plate according to the table below.
| Name of the Plate | Pre-loaded reagents | Addition before use |
| Sample plate | 500µl Buffer MLB 20µl MagPure Particle MPN | 200µl sample 10µl PK/Carrier RNA |
| Wash Plate 1 | 500µl Buffer MW1, Put in 96 magnetic Tip | |
| Wash Plate 2 | 500µl 80% ethanol | |
| Wash Plate 3 | 500µl 80% ethanol | |
| Elution plate | 50~100µl Nuclease Free Water | |
- Turn on the machine, start the corresponding program.
- Place the 96-well plate into the instrument as prompted.
- Finish the operation after ~20 minutes. Remove the 96-well plate and magnetic jacket.
- Store the Elute product at -20~8oC.
