Audited Supplier
HiPure MicroBiome DNA Kit
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D314802 | HiPure Microbiome DNA Kit | 50 preps | $348.00 |
| D314803 | HiPure Microbiome DNA Kit | 250 preps | $1,641.00 |
△ Introduction
The HiPure Microbiome DNA Kit provides a reliable solution for DNA enrichment and extraction of parasitic microorganisms.The kit is based on silica gel column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. The entire extraction process takes only 60 minutes.The kit is suitable for the enrichment and extraction of microbial DNA from anticoagulant blood, tissue and intestinal microorganisms, and the efficient removal of cellular DNA.The obtained DNA can be directly used for PCR, enzyme digestion, Southern blot and other experiments.
△ Details
| Features | Specifications |
| Main Functions | Isolation gDNA from biological sample and remove host DNA |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Culture medium, swab, parasitic blood, tissue, sputum, etc. |
| Sample amount | Blood sample, homogenate, plasma, brain effusion, swab immersion solution, centrifuged concentrated liquid, etc.:0.5-1ml |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
△ Advantages
- High concentration - the host DNA is digested by dnase, without contamination of host DNA
- High recovery - DNA can be recovered at the level of PG
- Good repeatability - silica technology can obtain ideal results every time
- Wide applicability - it can be used in blood, tissue, intestinal contents and other samples
△ Kit Contents
| Contents | D314802 | D314803 |
| Purification Times | 50 Preps | 250 Preps |
| Hipure DNA Mini Columns I | 50 | 2 x 125 |
| 2ml Collection Tubes | 50 | 2 x 125 |
| 2ml beads Tubes | 50 | 250 |
| Buffer DRB | 15 ml | 60 ml |
| Buffer ES | 6 ml | 30 ml |
| Reagent DX | 0.5 ml | 1 ml |
| Buffer DL | 30 ml | 120 ml |
| Buffer GW1 | 22 ml | 110 ml |
| Buffer GW2 | 12 ml | 50 ml |
| DNase I (Powder) | 6 mg | 30 mg |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Buffer AE | 15 ml | 60 ml |
△ Storage and Stability
DNase I and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
