Audited Supplier
D3182C
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Biochemical Reagents
Classic
For Biological Purpose, For Microbiological, For Refractive Index, Isolation Circulating DNA From 1-5ml Plasma, Serum
Standard
Laboratory Reagents, Diagnostic Reagents, Teaching Reagents, Qpcr, Liquid or Solid Chip Analysis, Hybridization
Laboratory Production
Circulating DNA MIDI Kit C (Vaccum Protocol
Industry, Scientific Research, Health, Environmental Protection, Agriculture, Isolation Circulating DNA From 1-5ml Plasma, Serum
Hipure Circulating DNA MIDI Kit C (Vaccum Protocol
Qpcr, Liquid or Solid Chip Analysis, Hybridization
Mini Spin Column
Silica Technology
Manual (Vacuum)
Serum, Plasma and Other Cell-Free Fluid Samples
1-5ml
≥50μl
≤60 Minutes
4ml
1mg
Free Sample
Technical Support
FedEx, DHL, Door to Door
Magen Biotech
Box
10 preps/ 50 preps
China
90279000
Product Description
HiPure Circulating DNA Midi Kit C (Vaccum Protocol)
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D318201C | HiPure Circulating DNA Midi Kit C | 10 preps | $136.00 |
| D318202C | HiPure Circulating DNA Midi Kit C | 50 preps | $547.00 |
△ Introduction
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. The extracted products can be used for clinical in vitro detection.
△ Details
| Features | Specifications |
| Main Functions | Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol |
| Applications | qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (vacuum) |
| Sample type | Serum, plasma and other cell-free fluid samples |
| Sample amount | 1-5ml |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 1mg |
△ Advantages
- High yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extent
- High concentration - low elution volume, ensuring high nucleic acid concentration
- High purity - low alcohol binding method, completely removing inhibitor and protein pollution
- High recovery - DNA can be recovered at thelevel of PG by silica gel column purification
△ Kit Contents
| Contents | D318201C | D318202C |
| Purification Times | 10 | 50 |
| Buffer ACL | 50 ml | 250 ml |
| Buffer ACB* | 60 ml | 300 ml |
| Buffer DCW1* | 4.4 ml | 22 ml |
| Buffer DCW2* | 5 ml | 10 ml |
| Proteinase K | 110 ml | 540 mg |
| Protease Dissolve Buffer | 10 ml | 30 ml |
| Carrier RNA | 110 μg | 110 μg |
| Nuclease Free Water | 10 ml | 20 ml |
| HiPure CFDNA Mini Columns | 10 | 50 |
| 2 ml Collection Tubes | 20 | 100 |
| Extender Tube | 10 | 50 |
| Vac-Connector | 10 | 50 |
△ Storage and Stability
Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
△ Experiment Data
