Audited Supplier
Product Name HiPure PCR Pure Mini Kit
Cat. No. & Specifications D212102,100Preps/kit
Introduction
HiPure PCR Pure Mini Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations,PCR,sequencing,restriction digestion,or various labeling reactions.In addition,this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Main Composition
| Product | D2121-02 |
| Purification Times | 100 Preps |
| Buffer DP | 60 ml |
| Buffer DW2 | 20 ml |
| Elution Buffer | 10 ml |
| HiPure DNA Mini Columns I | 100 |
| 2 ml Collection Tubes | 100 |
Storage conditions and Validity
HiPure Gel Pure DNA Kits components are guaranteed for at least one year when stored at room temperature.If any precipitates form in the buffers,warm at 37ºC to dissolve.
Materials and Equipment to be Supplied by User
Troubleshooting Guide
1. Low or no recovery
2. DNA does not perform well (e.g. in ligation reaction)
Eluate contains denatured ssDNA, which appears as smaller smeared band on analitical gel: Use the Eluted DNA to prepare the subsequent enzymatic reaction but omit the enzyme. To reanneal the ssDNA, incubate the reaction mixture at 95oC for 2 min, and allow the tube to cool slowly to room temperature. Add enzyme and proceed as usual.
