Audited Supplier
Key to this kit is our proprietary DNA binding systems that allow the high efficient binding of DNA to the Mini Column while proteins and other impurities are removed by Wash Buffer. Nucleic acids are then eluted with sterile water or Elution Buffer. This kit is designed for fast and efficient purification of plasmid DNA from 1 to 5 mL of E. coli culture. The Mini Column has a plasmid DNA binding capacity of 50 μg. The yield from 1 mL culture is typically around 8 to 12 μg. The purified DNA is ready for downstream applications such as cloning/subcloning, RFLP, sequencing, and transfection of robust cells such as HEK293 cells.
Catalog# | BW-PD1211-00 | BW-PD1211-01 | BW-PD1211-02 | BW-PD1211-03 |
Preps | 10 | 50 | 100 | 250 |
Mini Columns | 10 | 50 | 100 | 250 |
2 mL Collection Tubes | 10 | 50 | 100 | 250 |
Buffer A1 | 3 mL | 15 mL | 28 mL | 70 mL |
Buffer B1 | 3 mL | 15 mL | 28 mL | 70 mL |
Buffer N1 | 4 mL | 20 mL | 40 mL | 100 mL |
Buffer KB | 6 mL | 30 mL | 55mL | 135 mL |
DNA Wash Buffer* | 3 mL | 12 mL | 22 mL | 54 mL |
Elution Buffer | 2 mL | 10 mL | 30 mL | 30 mL |
RNase A (20 mg/mL) | 15 μL | 75 μL | 140 μL | 350 μL |
User Manual | 1 | 1 | 1 | 1 |
Test Principle The adsorption membrane within the column is a specialized silica gel membrane that exhibits specific nucleic acid adsorption properties.
RNase A: 20 mg/mL. It is stable for one year at room temperature (15-25). Spin down RNase A vial briefly before adding to Buffer A1. Buffer A1 should be stored at 4 once RNase A is added. Add 12 mL (BW-PD1211-00) or 48 mL (BW-PD1211-01) or 88 mL (BW-PD1211-02) or 216 mL (BW-PD1211-03) 96-100% ethanol to DNA Wash Buffer bottle before use. Buffer B1 precipitates below room temperature. It is critical to warm up the buffer at 37 to dissolve the precipitates before use. Keep the cap tightly closed for Buffer B1 after use. Ensure the availability of centrifuge capable of 12,000 rpm. Carry out all centrifugations at room temperature.
Materials not Supplied
High speed microcentrifuge or vacuum manifold. 96-100% ethanol. 1.5 mL microcentrifuge tubes.
Safety Information
Buffer N1 contains chaotropic salts, wear gloves and protective eyewear when handling. Buffer KB contains chaotropic salts, which may form reactive compounds when combines with bleach. Do not add bleach or acidic solutions directly to the waste.
Storage and Stability
Buffer A1 should be stored at 4 once RNase A is added. All other materials can be stored at room temperature (15-25). The guaranteed shelf life is 12 months from the date of production.
Hangzhou Beiwo Medical Technology Co., Ltd.
Beiwo was founded in 2018 and headquartered in Hangzhou, China with sister company, Biomiga Inc. located in San Diego, USA. Beiwo is based in the fields of molecular biology and diagnostics, and has a unique "product + service" model. Beiwo's partners include UWI, Genescript,Peking University, Tsinghua University, Zhejiang University, UCSD and other well-known institutions.
Beiwo has a R&D team consisting of experts from wordwide, and has established a high-standard 100,000-grade GMP production workshop and obtained the ISO9001 quality management system certification.
Based on the business philosophy of "trust comes from quality". Beiwo Medical focuses on global needs and is dedicated to providing customers with quality products and good technical services.
Q: What kinds of products do you have?
A: sample preparation and purification, analysis and detection, BEIWO has corresponding reagents, instruments and even automation platform for scientific research institutions, academic institutions and industrial customers in the biopharmaceutical industry.
Q: How can I get the sample to check your quality?
A: We can ship the sample by international express like FEDEX, DHL etc, or we can choose the appropriate express logistics for you according to your requirements. Sample and freight cost will be needed.
Q: Can you provide different specifications of the kits according to the quantity we need?
A: Yes, we can provide the kits according your requirements, and we also can do ODM and OEM.
Q: What's your MOQ?
A: The MOQ for kits is 10 boxes. If some samples are needed, the total number of samples cannot be less than 5 boxes. For customized products, you can set the scales with your specification, logo, packing, etc, so the MOQ shall be negotiated.
Q: Do you have any certificates?
A: Yes, we have the certificatesISO9001, CErequired for export and local import custom clearance.
Q: What's your production capacity?
A: We have the production capacity of 50 thousand tests of kits per month.
Q: What's your lead time?
A: Our lead time is 5-10 days, if you need a large amount, the lead time may be 7-15 days, if more, then the exact time can be negotiated.
Q: What are your method of payment?
A: We support L/C, D/P, or T/T to business account.
