Slu-PP-332 Manufacture/5-Amino-1mq/5 Amino 1mq/Nnmti 98% Peptide Raw Powder 42464-96-0

Product Name: NNMTi (5-amino-1MQ)
Chemical Name: 5-amino-1-methylquinolin-1-ium iodide
CAS Number: 42464-96-0
Appearance: Red Powder
Purity: 99%

Product Description:

NNMTi (5-amino-1MQ) is a potent and selective inhibitor of nicotinamide N-methyltransferase (NNMT), with an IC50 value of 1.2 μM. It binds selectively to substrate-binding site residues of NNMT. NNMTi has shown significant potential in promoting muscle cell differentiation in vitro and enhancing muscle stem cell fusion and regeneration in aged mice.

Applications:

1. Muscle Regeneration Research:

   • Enhances the differentiation of C2C12 myoblasts and supports muscle regeneration after injury.
   • Increases the proliferation and activation of muscle stem cells (muSCs).

2. Cell Biology and Aging Studies:

   • Useful for studying NNMT's role in aging-related muscle degeneration and cellular differentiation.

Mechanism of Action:

NNMTi inhibits nicotinamide N-methyltransferase activity, promoting differentiation and fusion of myoblasts while enhancing the regenerative ability of aged muscle stem cells.

In Vitro Studies:

• Myoblast Differentiation:
  • NNMTi (10-30 μM; 96 hours) induces a concentration-dependent increase in differentiation of C2C12 myoblasts.
  • At 30 μM, NNMTi leads to 18% MHC-positive myotube nuclei, representing a 45% increase compared to untreated cells.

In Vivo Studies:

• Muscle Regeneration in Mice:
  • Subcutaneous injection of NNMTi (5 mg/kg and 10 mg/kg for 2 weeks) significantly improves muscle regeneration post-injury.
  • Results in 60% and 75% higher incidence of proliferating/active muscle stem cells (muSCs) at 5 mg/kg and 10 mg/kg, respectively.
  • The number of fibers with EdU+ myonuclei increases by 40% and 48% at the respective doses.
  • No systemic toxicity was observed even after daily dosing for 1 week, with normal levels of glucose, cholesterol, plasma proteins, and electrolytes.

Key Features:

• Selective Inhibition: High specificity for NNMT with minimal off-target effects.
• Muscle Differentiation: Promotes myoblast fusion and differentiation effectively.
• Safe Profile: Well-tolerated in vivo with no systemic toxicity.

Storage Information:

• Store at -20°C in a sealed, dry container. Protect from moisture and light to maintain stability.

Use Cases:

• Regenerative Medicine: Investigating therapies for muscle regeneration and aging-related muscle disorders.
• Cell Signaling Studies: Exploring NNMT's role in cellular differentiation and metabolism.

This product is for research purposes only and not for human or veterinary use. For additional details or bulk inquiries, please contact us.