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Trihydroxymethylaminomethane is an organic compound with the molecular formula C4H11NO3. It is a white crystalline particle or powder that is easily soluble in water. Trihydroxymethylaminomethane is widely used in biochemical and molecular biology experiments. It can serve as a solvent for nucleic acids, proteins, and other biomolecules, helping to maintain the pH stability of the research solution. In addition, Tris can also be used to prepare surfactants, vulcanization promoters, and intermediates for some drugs.
| English name | Trometamol |
| Chinese name | Tris- hydroxymethyl-aminometh |
| Abbreviation | Tris |
| CAS# | 77-86-1 |
| Molecular weight | 121.135 |
| Molecular formula | C4H11NO3 |
| Storage condition | room temperature, away from light and moisture |
| Appearance | white crystal powder |
(Molecular structure)
the product can be used for the preparation of buffer solution in biochemistry and molecular biology experiment, pharmaceutical intermediate, surfactant and curing accelerator, water-soluble polymer containing Tris structure unit as paint dispersant, formaldehyde adsorption material in the room, etc.
1. Chemical raw material: Tris is widely used as an important chemical raw material for flame retardant materials, coatings and amino resins, etc.
2. Reaction medium: As an excellent reaction medium, Tris is often used in organic synthesis reactions, which can play the role of catalyst.
3. Resin cross-linking agent: Tris(hydroxymethyl)aminomethane can also be used in resin cross-linking reaction, which can improve the hardness and heat resistance of resin.
4. Biological buffering agent: In the field of medicine, Tris is used as a biological buffering agent, which is widely used in cell culture, DNA/RNA extraction, PCR amplification, etc.
1. Weigh the required amount of Tris powder and add it to sterile high purity water.
2. Use a magnetic stirrer to fully dissolve the Tris powder until it is completely homogeneous.
3. Calculation of concentration: Generally, the concentration of trimethylolaminomethane is 1 mol/L, and the concentration can be adjusted appropriately according to the need.
4. Measure the PH value of the solution with a PH meter, which should generally be controlled between 7.0-8.5.
5. Preparation of buffer solution: standardize the solution to the required volume, usually used as a buffer solution with a concentration of 50-200 mmol/L.
6. Sterilization: The trimethylolaminomethane solution is loaded into sterile bottles and sterilized according to a certain process.
It should be noted that during the configuration of trimethylolaminomethane, chemical safety operation procedures should be strictly observed, and personal protective equipment should be worn to avoid splashing the chemical on the skin and eyes, and to avoid inhalation and accidental ingestion of the chemical. At the same time, the label and production date of the chemical should be checked before use to avoid using expired or poor quality chemicals.
Each buffer developed has a specific buffer capacity and buffer range. Buffer capacity is the amount of acid or base that can be added before a significant change in pH begins. It can also be defined as the amount of strong acid or strong base that must be added to change the pH value of one liter of solution by one pH unit. After adding acidic or alkaline components, it plays a role in resisting pH changes. Maintain a relatively stable pH value of the solution by neutralizing a small amount of added acid or base.
Desheng is a manufacturer of industrial grade biological buffering agents, currently producing dozens of buffering agents that can be used in various industries.
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!
